P-227 ICSI outcomes after using in-situ microfluidics of fluidic walls versus DGC: a prospective non-inferiority comparative pilot study in sibling oocytes

Abstract Study question Does the novel strategy in-situ microfluidics (isM) yield comparable ICSI outcomes to control sperm selection methodology density gradient centrifugation (DGC)? Summary answer show that handmade of fluidic walls are as effective DGC achieve fertilization and usable day 5 blastocyst formation rate. What is known already Microfluidics technologies proving their capability select suitable for ICSI. Increasingly, publications ingenious strategies aimed integrate biomimicry during in vitro while simplifying IVF-workflow. We recently designed a system which allows selecting same ICSI-dish, only using disregarding centrifugation, washing plasticware. A previous proof-of-concept study showed this was efficient it could separate at least 20 progressive spermatozoa less than 15 minutes clean microdroplet free any remaining seminal plasma. design, size, duration The present pilot included total 280 fresh MII-oocytes allocated 1:1 ratio (isM-ICSI) (DGC-ICSI) groups. statistical power established 80% CI 95%. For comparison we relied KPIs: normal rate (INFR) Day (D5UBR). non-inferiority margin (Δ) 2-sigma warning limit our historical mean values (ΔINFR = 7% ΔD5UBR 10%). Participants/materials, setting, methods An informed consent signed by all participants. comparative 29 consecutive cases performed oocytes from women under 40 years old with an oocyte ≥6 MII. All semen samples had ≥1 × 106/ml were split into three aliquots: 1-seminogram (100 µl), 2-isM (10 3-DGC (surplus). Embryos cultured bench-top Time-lapse incubators, GTL-media 6,4% CO2 5% O2 conditions up stage. Main results role chance Sperm characteristics following concentration (42,83 ± 31,1 106/ml), motility (51,28 14,16 %) (17 12 106/ml). number MII per patient 9,66. From (n 280), 139 isM (study group) 141 (control INFR 72,0 % vs 76,6 %, respectively. Non-significant differences observed (p 0,54). blastocysts (day 5/6) 1,83 (53/29) 1,66 (48/29) DGC. Moreover, D5UBR 53,0% 44,4 0,26) difference between within margin. lower arrested embryos (up 6) group (19,0% 30,0%), although not statistically significant 0,08). Limitations, reasons caution This includes limited oocytes. protocol depends on free-hand preparation. Although use templates training reduces inter intra-operator variability, ready-to-use surface would benefit operability. Further investigation required evaluate clinical pregnancy live birth outcomes. Wider implications findings DNA fragmentation cited among causes embryo arrest. As resulted proportion embryos, exploring its capacity non-fragmented warrants further interest. streamlines ICSI, costs risks along procedure. Trial registration na